Western Palearctic


Within the Palearctic ecozone we have worked mainly in Central and Eastern Europe, Mediterranean region, Middle East, and North Africa. We have studied phylogeographic patterns, species diversifications, molecular taxonomy, or phenotype variation in various amphibian and reptile taxa, in the first place tree frogs (Hyla), slow-worm lizards (Anguis), and geckos (Hemidactylus).

Our research is commonly conducted in cooperation with colleagues from the Department of Zoology of the Natural History Museum, National Museum in Prague (Jiří Moravec, Jiří Šmíd), and Department of Zoology at the Comenius University in Bratislava, Slovakia (David Jandzík, Peter Mikulíček, Daniel Jablonski). Some (important ;-)) projects were carried out during my doctoral studies at the Institute of Animal Physiology and Genetics, Liběchov, Czech Academy of Sciences (under supervision of Petr Kotlík).

 

Research highlights

anguis_DSC_4260

Slow worm as a species complex

Semifossorial legless lizards from the genus Anguis, slow worm, had been understood as representing only two species till the end of the first decade of the 21st century – A. fragilis and A. cephallonica. Using phylogeographical approach we showed that A. fragilis represents more species, a species complex. Using genetic markers we distinguished as separate species the western populations (A. fragilis sensu stricto), eastern populations (A. colchica), and the species name A. graeca was applied for the southern Balkan populations. These findings were published in 2010 here. Later, an ancient lineage of slow worms was detected in the Italian Peninsula, named A. veronensis, and introduced together with a thorough morphological analysis in 2013 (see here). We hypothesize that the Tertiary Alpine orogeny with subsequent vicariance might have played a role in differentiation of this species. The current genetic variability was later shaped in multiple glacial refugia, with the first splitting event separating populations from the region of the Dolomite Mountains, thus, highlighting their biogeographical importance.

Anguis_kolaz

 

In 2016, we published an article studying distribution and genetic structure of mitochondrial lineages in the Balkans. Phylogeographic pattern is concordant with the refugia-within-refugia model. While slow-worm populations from the southern refugia mostly have restricted distributions and have not dispersed much from their refugial areas, populations from the extra-Mediterranean refugia in northern parts of the Balkans have colonized vast areas of eastern, central, and western Europe. We further found a strong correlation between genetic diversity of slow-worm populations and topographic ruggedness of the mountain systems they inhabit. Areas with more rugged terrain harbour higher genetic diversity.

Anguis_Balkansterrain_ruggedness

Jablonski D., Jandzik D., Mikulíček P., Džukić G., Ljubisavljević K., Tzankov N., Jelić D., Thanou E., Moravec J., Gvoždík V., 2016: Contrasting evolutionary histories of the legless lizards slow worms (Anguis) shaped by the topography of the Balkan Peninsula. BMC Evolutionary Biology 16: 99

Gvoždík V., Benkovský N., Crottini A., Bellati A., Moravec J., Romano A., Sacchi R., Jandzik D., 2013: An ancient lineage of slow worms, genus Anguis (Squamata: Anguidae), survived in the Italian Peninsula. Molecular Phylogenetics and Evolution 69: 1077–1092

Gvoždík V., Jandzik D., Lymberakis P., Jablonski D., Moravec J., 2010: Slow worm, Anguis fragilis (Reptilia: Anguidae) as a species complex: Genetic structure reveals deep divergences. Molecular Phylogenetics and Evolution 55: 460–472

We continue our research on the phylogeography and population genetics of slow worms with special interests in the situation in the Balkans and Central Europe. Our further aims are mainly to characterize still widely unknown distribution ranges, to detect contact zones of the parapatric species, and to test for the signature of introgressive hybridization using genomic data.

Read our publications.

 


Tree frogs

The Western Palearctic tree frogs (Hyla) are distributed from the Northwest Africa and Canary Islands, across Europe including the main Mediterranean Islands to the Iranian Highlands and south-western Arabian Peninsula. We have studied tree frogs from around the Western Palearctic range with special emphasis on the populations from the eastern Mediterranean and Middle East.

Morphological evolution

First studies were using morphological approach and compared populations of the Middle Eastern tree frog, H. savignyi, and eastern populations of what was H. arborea at that time. The analyses showed that the body shape is more similar in areas of similar climatic conditions, even when different species are compared, than in areas of different climate, even within the same species. Thus, in tree frogs the external morphology is more affected by environmental/climatic conditions than by genotype.

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 upgma

 

 

 

 

 

 

Middle Eastern tree frogs

Similarly, when we evaluated the colour pattern of the eastern Mediterranean tree frogs (H. savignyi) we found out that tree frogs from Cyprus are more frequently spotted than the mainland populations, however, as we tested later they are not differentiated genetically as they form the same haplogroup with the southern Turkish population. Within this phylogeographic study we detected distribution, evolutionary relationships and demographic history of several lineages of tree frog distributed within the Middle East and Caucasus. In addition, we also described a new species, H. felixarabica. Using bioacoustics tools we also identified H. orientalis for the first time in Iran.

Hyla_felixarabicaHyla_felixarabica_Fidan

Arabian Tree Frog (Hyla felixarabica) was described as a new species in 2010, and is known from two disjunct populations, from the south-western Arabian Peninsula and southern Levant (Jordan, southern Syria, and extreme north-eastern Israel).

 

 

Boulenger1898

Illustration of a Cypriote specimen of H. savignyi published by Boulenger (1898: The Tailless Batrachians of Europe. Part II. Ray Sociandy, London, Plate XV, Figure 3).

 

 

 

 

Widespread introgression in the European short-call tree frogs

Hyla_short-call

Finally, so far, we also studied the speciation history of the European short-call tree frogs, i.e. a group of the Western Palearctic tree frogs morphologically cryptic but genetically differentiated and characterized by short temporal parameters of the advertisement call (H. arborea, H. orientalis and H. molleri from across Europe to western Asia, two putative taxa within H. intermedia from the Italian Peninsula and Sicily, and H. sarda from Sardinia and Corsica). We inferred genealogical histories and species limits showing that gene introgression played an important role in the evolutionary history of the short-call tree frogs. A mitochondrial capture upon secondary contact appears to explain the close mtDNA relationship between the geographically remote Iberian H. molleri and H. orientalis from around the Black Sea.

 

Gvoždík V., Canestrelli D., García-París M., Moravec J., Nascetti G., Recuero E., Teixeira J., Kotlík P., 2015: Speciation history and widespread introgression in the European short-call tree frogs (Hyla arborea sensu lato, H. intermedia and H. sarda). Molecular Phylogenetics and Evolution 83: 143–155

Gvoždík V., Moravec J., Klütsch C., Kotlík P., 2010: Phylogeography of the Middle Eastern tree frogs (Hyla, Hylidae, Amphibia) as inferred from nuclear and mitochondrial DNA variation, with a description of a new species. Molecular Phylogenetics and Evolution 55: 1146–1166

Gvoždík V., 2010: Second species of tree frog, Hyla orientalis (formely H. arborea), from Iran confirmed by acoustic data. Herpetology Notes 3: 41–44

Gvoždík V., Moravec J. & Kratochvíl L., 2008: Geographic morphological variation in parapatric Western Palearctic tree frogs, Hyla arborea and Hyla savignyi: are related species similarly affected by climatic conditions? Biological Journal of the Linnean Society 95: 539–556

Gvoždík V., Moravec J., 2005: Variation of Hyla savignyi: A color pattern of Cypriote and mainland populations. In: Ananjeva N. & Tsinenko O. (Eds.): Herpetologia Petropolitana. Proc. of the 12th Ord. Gen. Meeting Soc. Eur. Herpetol., August 12–16, 2003, St. Petersburg. Russian Journal of Herpetology 12 (Suppl.): 32–34

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Arid Hemidactylus Geckos

Hemidactylus is a speciose gecko genus with more than 130 species, circumtropical (and subtropical) almost worldwide distribution, and is formed by several main clades. The Arid clade is represented by more than 50 species occurring in arid habitats of the north-eastern Africa, Horn of Africa, Arabian Peninsula and western Asia. This includes also the single circum-Mediterranean species, and the only European species, H. turcicus. We initially focused on populations from the Mediterranean, Syrian Desert and Arabia with the aim to find out the phylogenetic position of H. t. lavadeserticus. Based on the molecular data and morphology, we not only elevated H. t. lavadeserticus to a full species but more importantly discovered a new species distributed in Jordan and southern Syria, which we named H. dawudazraqi. We also suggested the existence of a possible new taxon in north-eastern Israel, and highlighted an overlooked extremely high diversity in the Arabian Peninsula, including a presence of an unnamed form in Sinai.

Hemidactylus_turcicus

 

Hemidactylus turcicus Hemidactylus_tree

 

This study was a stepping stone for a series of subsequent studies (performed in the course of the PhD study of Jiří Šmíd), which detailed the taxonomy and biogeography of the Arabian, Near Eastern, and north-east African populations and brought descriptions of further four species (H. adensis, H. mandebensis, H. ulii from Yemen, and H. awashensis from Ethiopia), and a revalidation of one species (H. granosus from Sinai, and Saudi Arabia).

 

Hemidactylus_map

 

 

 

The molecular data allowed an inference of the evolutionary and biogeographical history of the Arid clade. Two basal divergences correspond with the break-ups of the Arabian and African landmasses and subsequent separation of the Socotra Archipelago from the Arabian mainland, respectively. Formation of the Red Sea led to isolation and subsequent radiation in the Arabian Peninsula, which was followed by multiple independent expansions: to present-day Iran, two colonizations to NE Africa, two to Socotra, and two to the Near East. Our study also highlighted the role of Arabia and the Horn of Africa as an important Hemidactylus diversity hotspot.

 

Moravec J., Kratochvíl L., Amr Z.S., Jandzik D., Šmíd J., Gvoždík V., 2011: High genetic differentiation within the Hemidactylus turcicus complex (Reptilia: Gekkonidae) in the Levant, with comments on the phylogeny and systematics of the genus. Zootaxa 2894: 21–38

Šmíd J., Carranza S., Kratochvíl L., Gvoždík V., Nasher A.K., Moravec J., 2013: Out of Arabia: A complex biogeographic history of multiple vicariance and dispersal events in the gecko genus Hemidactylus (Reptilia: Gekkonidae). PLoS ONE 8 (5): e64018

Šmíd J., Moravec J., Kratochvíl L., Gvoždík V., Nasher A.K., Busais S.M., Wilms T., Shobrak M.Y., Carranza S., 2013: Two newly recognized species of Hemidactylus (Squamata, Gekkonidae) from the Arabian Peninsula and Sinai, Egypt. ZooKeys 355: 79–107

Šmíd J., Moravec J., Kratochvíl L., Nasher A.K., Mazuch T., Gvoždík V., Carranza S., 2015: Multilocus phylogeny and taxonomic revision of the Hemidactylus robustus species group (Reptilia, Gekkonidae) with descriptions of three new species from Yemen and Ethiopia. Systematics and Biodiversity 13 (4): 346–368

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Snakes (Vipera, Zamenis, Natrix)

Endangered meadow vipers (Vipera ursinii complex)

The last two populations of the Hungarian meadow viper Vipera ursinii rakosiensis were thought to persist in the steppe fragments of Hungary until meadow vipers were discovered in central Romania (Transylvania), suggesting a possible existence of remnant populations elsewhere. We assessed the phylogenetic position of the Transylvanian vipers using mitochondrial DNA. We showed that they were closely related to the Hungarian vipers, while those from northeastern Romania (Moldavia) and Danube Delta belonged to the subspecies Vipera ursinii moldavica. Montane subspecies from Europe (Vipera ursinii ursinii and Vipera ursinii macrops) formed a sister clade to the two lowland subspecies. Vipera renardi formed a sister clade to V. ursinii, with populations from the Greater Caucasus (Vipera renardi lotievi) and Tien Shan (Vipera renardi tienshanica) as the sister group to Vipera renardi renardi, and Vipera renardi eriwanensis from the Lesser Caucasus as the most basal taxon in the species. Our results illustrated that the divergence between the lowland and montane populations occurred separately in each species and several times in V. renardi. In our publication in 2012, we demonstrated that the recently discovered Transylvanian population is the third surviving population of V. u. rakosiensis and the only known population outside of Hungary.

V_ursinii_mapV_ursinii

Vipera ursinii rakosiensis from Romanian Transylvania

 

 

 

 

 

 

Two subspecies and three lineages of a rare snake, Zamenis hohenackeri

Two morphologically differentiated subspecies with presumably allopatric distribution have traditionally been recognized in the rare Transcaucasian rat snake, Zamenis hohenackeri: Z. h. hohenackeri (Caucasus region and E Turkey, NW Iran and N Iraq) and Z. h. tauricus (S Anatolia and Levant). Both subspecies are sometimes considered just colour forms of a monotypic species. We used mitochondrial DNA to find out whether both subspecies are also genetically differentiated. We found that the species forms not two but three divergent lineages: one corresponds to the nominotypic subspecies, another comprises an individual from the region of the type locality of Z. h. tauricus in southern Anatolia and the third, with no available name and separated from the previous by the Amanos (Nur) Mountains, is distributed in the Levant. The pattern of divergence and distribution of the lineages correspond to other species of amphibians and reptiles, demonstrating that similar geo-climatic events presumably formed genetic variation and triggered speciation processes in this region.

This research was led by David Jandzík, Comenius University, Bratislava, Slovakia.

Zamenis_hohenackeri_Armenia_Roberto_SindacoZamenis_hohenackeri_tauricus_Mugla_Turkey_Konrad_Mebert

Z. h. hohenackeri from Armenia on left (photo by R. Sindaco), Z. h. tauricus from SW Turkey on right (photo by K. Mebert)

 

Phylogeography, contact zones and taxonomy of Natrix natrix

Grass snakes (Natrix natrix) represent one of the most widely distNatrix_natrix_phylogeoributed snake species of the Palaearctic region, ranging from Northwest Africa and the Iberian Peninsula through most of Europe and western Asia eastward to the region of Lake Baikal. Within N. natrix, up to 14 distinct subspecies were regarded as valid. In addition, some authors recognized big-headed grass snakes from western Transcaucasia as a distinct species, N. megalocephala. We presented a nearly range-wide phylogeography for both nominal species. Within N. natrix, 16 terminal mitochondrial clades were identified, most of which conflicted with morphologically defined subspecies. These 16 clades corresponded to three more inclusive clades from (i) the Iberian Peninsula plus Northwest Africa, (ii) East Europe and Asia and (iii) West Europe including Corso-Sardinia, the Apennine Peninsula and Sicily. In Central Europe, two contact zones of three distinct mitochondrial clades were uncovered, which was quite unexpected situation. In agreement with previous studies using morphological characters and allozymes, we found no evidence for the distinctiveness of N. megalocephala. Therefore, we synonymized N. megalocephala with N. natrix.

This research was primarily performed by Carolin Kindler and led by Uwe Fritz, Museum of Zoology, Dresden, Germany.

Natrix_natrix_Celilabad

Natrix natrix from SE Azerbaijan

 

Gvoždík V., Jandzik D., Cordos B., Rehák I., Kotlík P., 2012: A mitochondrial DNA phylogeny of the endangered vipers of the Vipera ursinii complex. Molecular Phylogenetics and Evolution 62: 1019–1024

Jandzik D., Avcı A., Gvoždík V., 2013: Incongruence between taxonomy and genetics: three divergent lineages within two subspecies of the rare Transcaucasian rat snake (Zamenis hohenackeri). Amphibia-Reptilia 34: 579–584

Kindler C., Böhme W., Corti C., Gvoždík V., Jablonski D., Jandzik D., Metallinou M., Široký P., Fritz U., 2013: Mitochondrial phylogeography, contact zones and taxonomy of grass snakes (Natrix natrix, N. megalocephala). Zoologica Scripta 42 (5): 458–472

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Paedomorphic newts

Facultative paedomorphosis is an environmentally induced polymorphism that is well known for many caudate species including newts. Although facultative paedomorphosis has been documented in some smooth-newt populations, records of entirely paedomorphic populations outside the Balkans are limited. We found and reported on a unique paedomorphic population of the smooth newt (Lissotriton vulgaris) in Central Bohemia, Czech Republic, and discussed potential causes of this paedomorphosis.

 newt_paedomorph

Gvoždík V., Javůrková V., Kopecký O., 2013: First evidence of a paedomorphic population of the smooth newt (Lissotriton vulgaris) in the Czech Republic. Acta Herpetologica 8 (1): 53–57

 

 

 

 

 

 


Václav Gvoždík, Research on the Evolutionary biology of amphibians & reptiles | Last updated July 2017